Part:BBa_K730001:Design

The mxaF gene is approximately 1.8 kb in size and encodes a 66-kDa polypeptide.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 120
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 120
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 120
  Illegal BamHI site found at 816
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 120
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 120
  Illegal NgoMIV site found at 447
  Illegal NgoMIV site found at 585
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Yes selected 1kb upstream region

Source

GCAAGGTACCGCCGTTCCTCTTCCTGAATCGCCAGTGAGCGTTGGGCCAGCGCACGGTTT TCCTCGCGGGTTTTTTCCAGCGTGCCGACCGTGTGGTTGAAGGCCCTCGAAATGCGGGCG AATTCCGGCAGGTAGAACTCCGGCAGGCGGCGGCTGTAATCACCGTTTTCCAGGTCTTCC AGCCCTTCGAGGATGATGCCCACGGATTTGAACGCCCGGCCCAGGGTGGTGTGGACGACC AGGTACACCGTGACGGCGAGTGCCACCATCAGCAGCAGAAACCCCCGTGCTTCGATCCAG GCCTCGGCGATCTCGTCGGCCGGGTTGGCGTCAACGGTGATCTGGATCATCGGGTTGCCG ATCTGGTGCAGATGTTTCTCCGCCACCAGGGGGTCCGGCGCGACGGCCCAGGCGAACCAG GCGGGCGCTTCCTCACCCTCCGCGGGGCCGGCAGAGGAGGGCAGATCGATGATCGTCCTT TCGCCTTGATGGATGCTGAGGCTCAGGTGGCGGGCGTGGTCGAGCCGGGCGAGCTGGGCG ATCCATTCGACCGGCGAGCGACCCGCCGCCACCGCCTGTTCCAGGCCGGCATCGATCAGC TGTAACGCCAGGTTGACCGATGACCGGACTTCCTCGGCCACCGACCGGCGCGCGTTGTGG ACCACGAACAGGGTGCCCATGCCGATGATGGCCAGCATGACCAGACCGATCATGAGGTTC AGGCGAAACCGCAGACTCAAGGGCGCTTGCTCCCGGGAAAGATCGTATTAGTTTGCCTCG ATCGGCGGTCCTTGTGACAGGGAGATATTCCCGACGGATCCGGGGCATTCGAGCGGAACC GCCCGCCGTGGGAGTTTTTCCAGCGAGCATTCGAGAGTTTTTCAAGGCGGCTTCGAGGGG TTATTCCGTAACGCCGCCGACATGATCTGTCCCAGAATCTCCGCCGCTGTTCGTAGAGCG CCGATGCAGGGTCGGCATCAATCATTCTTGGAGGAGACAC